An identical observation was designed for anti-MSP3 IgG3 replies where in fact the adults had significantly higher replies than both small children and teenagers (Dunns Multiple Evaluation Check, p?

An identical observation was designed for anti-MSP3 IgG3 replies where in fact the adults had significantly higher replies than both…

The splenic T lymphocytes were enriched with nylon wool columns, and 100 l of 2 106 cells/ml in RPMI-5 culture medium (RPMI 1640 containing 5% fetal bovine serum, 100 nM l-glutamine, 10 nM penicillin-streptomycin, and 5 10?5 M 2-mercaptoethanol) was added to each well in 96-well plates

The splenic T lymphocytes were enriched with nylon wool columns, and 100 l of 2 106 cells/ml in RPMI-5 culture…

FACS analysis was carried out using Fpro0165 IgG, and a mouse and a mouse anti-human FPR2 antibody (unpublished in-house reagent) anti-human CXCR3 antibody (R&D Systems, MAB160) with detection using either a PE-labeled anti-human IgG (Sigma P8047) or a PE-labeled anti mouse IgG1(Jackson ImmunoResearch,115-116-146) as appropriate

FACS analysis was carried out using Fpro0165 IgG, and a mouse and a mouse anti-human FPR2 antibody (unpublished in-house reagent)…