Supplementary horseradish peroxidase (HRP)-conjugated antibodies (1:2000; Invitrogen, Carlsbad, USA; “type”:”entrez-nucleotide”,”attrs”:”text”:”G21040″,”term_id”:”1341366″,”term_text”:”G21040″G21040, “type”:”entrez-nucleotide”,”attrs”:”text”:”G21234″,”term_id”:”1341560″,”term_text”:”G21234″G21234) were utilized based on the producers guidelines, and binding…