B
B.1980. culture fluid was removed, and flesh culture fluid was added into each well. Following five-day incubation, the culture fluid was added into equal volume of 0.5% porcine erythrocytes in a 96-well V-bottom microplate. The antibody titer was calculated as the highest serum dilution at 50% inhibition of hemagglutination. The antibody titer was analyzed statistically by the use of spreadsheet program (Microsoft Excel 2016). Anti-FPV HI antibody was not detected (titer of <1:10) in 53 (7.4%) of a total of 718 cats, and the HI antibody titer was 1:10, 1:20, and 1:40 Elastase Inhibitor in 15, 28, and 57 cats, respectively. These 153 cats were also examined for anti-FPV SN antibody. As shown in Fig. 1, the SN antibody was detected (1:10) in 33 (62.3%) of the 53 HI antibody-negative cats. The SN antibody titer in the HI antibody-negative cats ranged from <1:10 to 1 1:160, and the geometric mean (GM) was 1:20.4. As shown in Fig. 2, the SN antibody was detected (1:10) in all cats with the HI antibody titers FRPHE of 1 1:10, 1:20, and 1:40, and the SN antibody titer was distributed within ranges of 1 1:14C1:190 (GM: 1:70.4), 1:80C1:640 (GM: 1:267.6), and 1:130C1:1,500 (GM: 1:548.8), respectively. In analysis, an antibody titer of <1:10 was arbitrarily regarded as 1:5. The SN antibody titer exceeded 1:32, which is the highest among previously reported protective threshold, in all cats with the HI antibody titers of 1 1:20 or higher, whereas it was lower than this threshold in 56.6% (30/53) and 13.3% (2/15) of cats with the HI antibody titers of <1:10 and 1:10. Open in a separate window Fig. 1. Anti-FPV SN antibody titer in 53 HI antibody-negative cats (titer of <1:10). An arrow indicates the protective SN antibody titer threshold (1:32). Open in a separate window Fig. 2. Anti-FPV SN antibody titer in 15, 28, and 57 cats with the HI antibody Elastase Inhibitor titers of 1 1:10, 1:20 and 1:40, respectively. Gray, black, and white bars indicate the HI antibody titers of 1 1:10, 1:20 and 1:40, respectively. An arrow indicates the protective SN antibody titer threshold (1:32). The averages and standard deviations of the logarithmic value of the reciprocal SN antibody titer in Elastase Inhibitor the groups with the HI antibody titers of <1:10, 1:10, 1:20, and 1:40 were 1.309 0.548, 1.848 0.296, 2.427 0.237, and 2.739 0.244, respectively, and the coefficients of variation (CV) were 41.9, 16.0, 9.8, and 8.9%, respectively (Fig. 3). The Pearsons correlation coefficient (R) was 0.860, showing a slightly high correlation between the two antibody titers, but the CV value clarified the presence of variation (41.9%) in cats with the HI antibody titer of <1:10. The gender, age, breed, and days from the most recent vaccination of the test cats were also investigated, but no significant difference was noted in any item (test 159: 1239C1247. doi: 10.1007/s00705-013-1914-1 [PMC free article] [PubMed] [CrossRef] [Google Scholar] 3. Dhein C. R., Gorham J. R.1986. Host response to vaccination. 16: 1227C1245. doi: 10.1016/S0195-5616(86)50139-X [PubMed] [CrossRef] [Google Scholar] 4. Fastier L. B.1968. Feline panleucopeniaa serological study. 83: 653C654. doi: 10.1136/vr.83.25.653 [PubMed] [CrossRef] [Google Scholar] 5. Greene C. E.2012. Feline enteric viral infections. pp. 80C91. 53: 283C293. doi: 10.1016/S0378-1135(96)01225-4 [PubMed] [CrossRef] [Google Scholar] 7. Jakel V., Cussler K., Hanschmann K. M., Truyen U., K?nig M., Kamphuis E., Duchow K.2012. Vaccination against Feline Panleukopenia: implications from a field study in kittens. 8: 62. doi: 10.1186/1746-6148-8-62 [PMC free article] [PubMed] [CrossRef] [Google Scholar] 8. Johnson R. H.1971. Serologic procedures for the study of feline panleukopenia. 158: 2, 876. [PubMed] [Google Scholar] 9. King D..