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doi: 10.1371/journal.ppat.1001028. shown as blue boxes in the neighbor-joining trees. Red tick marks denote nonsynonymous amino acid mutations, and green tick marks denote synonymous amino acid mutations in the Highlighter plots. Cefadroxil Download FIG?S2, PDF file, 0.5 MB. Copyright ? 2020 Martinez et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. TABLE?S2. HIV Env binding profile of small-scale transient transfection of monoclonal IgG isolated from nontransmitting and transmitting HIV-infected U.S. and Malawian Cefadroxil women. Download Table?S2, XLSX file, 0.1 MB. Copyright ? 2020 Martinez et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. FIG?S3. Plasma and Env-specific IgG autologous-virus neutralization activity against nontransmitted maternal viruses in nontransmitting HIV-infected mothers. HIV Env-specific IgG MAbs isolated from nontransmitting mothers are shown in the blue font. Download FIG?S3, PDF file, Cefadroxil 0.02 MB. Copyright ? 2020 Martinez et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. FIG?S4. Plasma and Env-specific IgG autologous-virus neutralization activity against nontransmitted maternal viruses and their paired infant-T/F virus in transmitting HIV-infected mothers. HIV Env-specific IgG MAbs isolated from transmitting mothers are shown in the red font. The variants that were closest to the most genetically distinct maternal variants in relation to each paired infant-T/F virus are arranged from left to right, as shown by the arrow. Download FIG?S4, PDF file, 0.03 MB. Copyright ? 2020 Martinez et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. FIG?S5. Fine-epitope specificity of maternal linear V3-specific IgG MAbs with autologous-virus-neutralizing activity. (A) Amino acid sequence alignment of Rabbit polyclonal to cyclinA V3 peptide mutant library. (B to D) Fine specificity of V3-specific IgG MAbs isolated from a nontransmitting woman and from two transmitting women. High binding strength is shown in black, medium binding strength is shown in orange, and low binding strength is shown in red. V3-specific IgG MAbs were tested at 5?g/ml and were serially diluted threefold. V3 loop amino acid residues important for binding for each V3-specific IgG MAb are shown in blue boxes. Download FIG?S5, PDF file, 0.1 MB. Copyright ? 2020 Martinez et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. FIG?S6. Gp120/gp41 interface glycan bNAb mapping in transmitting HIV-infected woman 3902. (A) Woman 3902 plasma was tested against the gp120-gp41 interface glycan-ablating mutant viruses Ce1176 N88A, Ce1176 N625A, CD4 binding site glycan-ablating mutant virus Ce1176 N276Q, and V3 glycan-ablating mutant virus Ce1176 N332A. Download FIG?S6, PDF file, 0.01 MB. Copyright ? 2020 Martinez et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. FIG?S7. Env V1V2 genetic signatures in amplicons of mother-infant pair 0601. (A) Amino acid residue deletions and mutations within the V1V2 loop in infant-T/F virus and the closest nontransmitted maternal variant that are differentially neutralization sensitive to V2 glycan-targeting bNAbs. (B) Mapping of maternal-plasma PG9-like bNAb activity in transmitting HIV-infected woman 0601 against mutant viruses that ablate PG9-like bNAb activity. (C) The PG9-blocking activity of PG9-IgG1 MAb (positive control), a seronegative sample, and transmitting HIV-infected woman 0601. (D) Structural modeling of Cefadroxil the molecular interactions of PG9 and differentially neutralization-sensitive maternal and infant-T/F viruses. Download FIG?S7, PDF file, 0.2 MB. Copyright ? 2020 Martinez et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. FIG?S8. Signature sequence analysis of PG9 neutralization-resistant and -sensitive variants. (A) Amino acid Cefadroxil residue at position 732 in uniformly neutralization-resistant woman 9105 nontransmitted maternal variants. (B) Amino acid residue positions in woman 0155 nontransmitted maternal variants that were differentially neutralization resistant to PG9. Download FIG?S8, PDF file, 0.01 MB. Copyright ? 2020 Martinez et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. Efforts to eliminate MTCT of HIV with antiretroviral therapy (ART) have met little success, with >180,000 infant infections each year worldwide. It is therefore likely that additional immunologic strategies that can synergize with ART will be required to eliminate MTCT of HIV. To this end, understanding the role of maternal HIV Env-specific IgG antibodies in the setting of MTCT is crucial. In this study, we.