Orexin1 Receptors Posted on December 17, 2024 The splenic T lymphocytes were enriched with nylon wool columns, and 100 l of 2 106 cells/ml in RPMI-5 culture medium (RPMI 1640 containing 5% fetal bovine serum, 100 nM l-glutamine, 10 nM penicillin-streptomycin, and 5 10?5 M 2-mercaptoethanol) was added to each well in 96-well plates The splenic T lymphocytes were enriched with nylon wool columns, and 100 l of 2 106 cells/ml in RPMI-5 culture… The splenic T lymphocytes were enriched with nylon wool columns, and 100 l of 2 106 cells/ml in RPMI-5 culture medium (RPMI 1640 containing 5% fetal bovine serum, 100 nM l-glutamine, 10 nM penicillin-streptomycin, and 5 10?5 M 2-mercaptoethanol) was added to each well in 96-well plates Read More