The splenic T lymphocytes were enriched with nylon wool columns, and 100 l of 2 106 cells/ml in RPMI-5 culture medium (RPMI 1640 containing 5% fetal bovine serum, 100 nM l-glutamine, 10 nM penicillin-streptomycin, and 5 10?5 M 2-mercaptoethanol) was added to each well in 96-well plates

The splenic T lymphocytes were enriched with nylon wool columns, and 100 l of 2 106 cells/ml in RPMI-5 culture…

FACS analysis was carried out using Fpro0165 IgG, and a mouse and a mouse anti-human FPR2 antibody (unpublished in-house reagent) anti-human CXCR3 antibody (R&D Systems, MAB160) with detection using either a PE-labeled anti-human IgG (Sigma P8047) or a PE-labeled anti mouse IgG1(Jackson ImmunoResearch,115-116-146) as appropriate

FACS analysis was carried out using Fpro0165 IgG, and a mouse and a mouse anti-human FPR2 antibody (unpublished in-house reagent)…

The reason for this slight discrepancy between theoretical and actual CE measured values for G0-F is not clear, but could be attributed to variations in the efficiency of PNGase F digestion or the quantum efficiency of the fluorophore used for CE, which was conjugated in close proximity to the fucose

The reason for this slight discrepancy between theoretical and actual CE measured values for G0-F is not clear, but could…