The ethics committee has the same number as the approved study (35-9185
The ethics committee has the same number as the approved study (35-9185.81/G-59/09). (DAB-positive signal, middle images) in neighbour slices whereas negative controls showed no DAB signal (right images). adipose tissue-derived stem cell, 3,3′-diaminobenzidine, Prussian blue. (TIF 7971 kb) 13287_2017_545_MOESM4_ESM.tif (7.7M) GUID:?E5F29817-704C-4F96-AACD-DAEE18523E37 Data Availability StatementAll dataset(s) supporting the conclusions of this article are included within the article. Abstract Background In the field of experimental stem cell therapy, intra-arterial (IA) delivery yields the best results concerning, for example, migrated cell number at the targeted site. However, IA application also appears to be associated with increased mortality rates and infarction. Since many rodent studies systemically apply 1??106 cells, this could also be a consequence of engrafted cell number. The aim of this study was therefore to investigate the effect of different doses of adipose tissue-derived stem cells (ASCs) on engraftment rates and stroke outcome measured in vivo using 9.4-T high-field magnetic resonance imaging (MRI). Methods Male Wistar rats (test was chosen for calculation of statistical comparisons. A value 0.05 was considered significant. Results MRI analysis of ASC-derived signals 48 h post MCAo Forty-eight hours after IA transplantation, ASCs were distributed discretely throughout the entire lesion area in the ipsilateral side of the brain as shown by hypointense dots in representative T2*-weighted images. The intensity of the hypointense signal increased visibly with the number of cells injected (Fig.?1a). To quantify this effect, the mean CSI was calculated for each animal to evaluate differences in CSI between the ischaemic and non-ischaemic hemisphere (for data range, medians, and IQRs for CSI, see Table?1). Results showed that MCAo duration did not affect CSI for the control group and the group receiving 3??105 cells. For animals receiving 1??106 cells, the occlusion times could not be compared due to restricted data for the group undergoing MCAo for 90 min; however, due to a similar CSI in the first-mentioned organizations, a statistical assessment was performed for each and every group individually of occlusion time. CSI ideals were significantly lower for control animals than for animals treated with 5??104 cells (adipose cells derived stem cell, difference in cell signal intensity, middle cerebral artery occlusion Table 1 Group sizes, data range, median, and IQR for CSI and infarct sizes obtained 48 h post MCAo adipose tissue-derived stem cell, difference in cell signal intensity, interquartile range, middle cerebral artery occlusion MRI analysis of infarct size 48 h post MCAo To evaluate the effect of ASC engraftment on infarction, T2-weighted Hydrocortisone buteprate MR images were from the same animals and MRI slices. Forty-eight hours after MCAo and ASC injection, the lesion area in the ipsilateral part of the brain was visible like a hyperintense transmission and improved visibly after engraftment of 1 1??106 cells (Fig.?2a). Open in a separate windowpane Fig. 2 MRI analysis of infarct size 48 h post-MCAo. a Representative T2-weighted images showing infarction 48 h post MCAo are the related images to the T2*-weighted images demonstrated in Hydrocortisone buteprate Fig.?1 and are derived from the same MRI slices and animals. b Variations in infarct quantities acquired 48 h post MCAo are demonstrated for those treatment groups. Organizations are the same as in Fig.?1. The median for each group is definitely indicated like a adipose cells – derived stem cell, difference in cell signal intensity, middle cerebral artery occlusion To evaluate the effect of ASC engraftment on infarct size, the infarct quantities were identified as a percentage of the individual total brain volume (data range, medians, and IQRs for infarct size are demonstrated in Table?1). The results revealed no influence of occlusion time on infarct volume in the control group and the group receiving 3??105 FNDC3A cells. For animals receiving 1??106 cells, the occlusion times could not be compared due to restricted data for the group undergoing MCAo for 90 min; however, due to similar infarct quantities Hydrocortisone buteprate in.